[12] Singlet Oxygen Induces Fluorescent Proteins Dimerization

ChemBioChem 2010, 11, 2384.

Lights out at two: The oxidation of avGFP and EGFP by singlet oxygen not only diminishes their fluorescence emission and changes their absorption properties, but also generates undesired protein aggregation. This could have important consequences in studies in which pairs of fluorescent proteins are used, such as in FRET experiments.